The result of the analysis showed that high tumor budding grade ahead of the invasive front was positively correlated with late TNM stage (Spearman’s correlation, r = 0.3760, p < 0.0001) and high expression level of NAV2 (Fig. 2b, Spearman’s correlation, r = 0.6208, p < 0.0001). This evidence concerns the gene NAV2 and neoplasm.