Based on the findings that miRNA-506 expression was down-regulated in colon cancer tissues and was inversely associated with EZH2 expression, advanced clinical stage and lymph node metastasis, in vitro and in vivo experiments confirmed that miR-506 overexpression significantly inhibited cell proliferation and metastasis and that EZH2 is a direct target of miR-506. The gene discussed is EZH2; the disease is malignant colon neoplasm.