Since ST6GalNAc1 expression has been shown to increase establishment of metastases in breast and colon cancer [20, 21], we next examined tumour cell dissemination by injecting DU145 cells tagged with Gaussia luciferase (G-Luc) intravenously (Supplementary Figure 10A). This evidence concerns the gene ST6GALNAC1 and malignant colon neoplasm.