Flow cytometry analysis showed a significant increase in the percentage of ALDH+ cells from 10.50 ± 2.39% to 25.40 ± 3.33% (p < 0.01) in HT-1376 cells and from 15.67 ± 2.49% to 32.00 ± 4.93% (p < 0.05) in UM-UC3 cells after 24 h of treatment with 10 μM CIS (Figure 4). Here, LDHA is linked to in situ carcinoma.