HT-1376 and UM-UC3 BC cell lines were allowed to growth in matrigel coated plates with serum-free DMEM/F12 medium supplemented with basic fibroblast growth factor (bFGF), human recombinant epidermal growth factor (EGF) and B27 to assess the presence of putative CSCs. This evidence concerns the gene FGF2 and breast cancer.