This study demonstrated up-regulated active Rac1, CDC42 and RhoA expression in Hes1-expressing colon cancer cells, whereas down-regulated active Rac1, CDC42 and RhoA expression in Hes1-silencing cells, which indicated that Hes1 induced cytoskeleton reconstruction of colon cancer cells. This evidence concerns the gene RAC1 and malignant colon neoplasm.