HES1 and cancer: In addition, the localization of Hes1 was found in both the nucleus and cytoplasm of NP69, CNE2, and SUNE1 cells, based on immunofluorescence assay (Supplementary Figure S1), and the distribution of Hes1 was detected in the nucleus and cytoplasm of cancer cells based on IHC (Figure 1A, 1C).