AGO2 and infection: To determine the effect of Ago2 and HuR knockdown on viral RNA synthesis, cells expressing shAgo2, shHuR, or shAgo2+shHuR were infected with EV71 at an MOI = 2 and were cultured with 2 μg/ml actinomycin D. Viral positive-strand RNA levels were examined by real time RT-PCR 24 h post infection.