For this purpose we generated human colon cancer stable cell lines overexpressing either ACSL1, ACSL4 or SCD (ACSL1 cells, ACSL4 cells, and SCD cells, respectively) or the three genes simultaneously (x3 cells), transducing the DLD-1 CRC cell line with specific lentiviruses. This evidence concerns the gene ACSL1 and colonic neoplasm.