TSC1 and tuberous sclerosis: To generate an eye-specific TSC animal model, we used a loss-of-function approach by breeding mice carrying a conditional Tsc1 allele (Tsc1tm1Djk; referred to as Tsc1+/f or Tsc1f/f) with mice harbouring an Lhx2-promoter-driven Cre-recombinase transgene [Tg(Lhx2-Cre)1Lcar; referred to as Lhx2-Cre] (Hägglund et al., 2011; Uhlmann et al., 2002).