To further validate the expression of GPC6 and TMEM132D at protein level in the cancer cells, we performed immunohistochemistry in sections from representative tumor samples with either high or low mRNA levels of GPC6 and TMEM132D as categorized by qPCR (Figures 1(e)-1(f) and 2(e)-2(f)). The gene discussed is GPC6; the disease is neoplasm.