Recently, we have shown that ANO6 is expressed in cyst-forming epithelial cells together with ANO1, which is widely expressed in epithelial cells.13 Knockdown of ANO1 but not ANO6 significantly reduced secretion-dependent cyst growth pointing towards distinct functions of ANO1 and ANO6 in the cyst epithelium.13 This evidence concerns the gene ANO6 and cyst.