ANO6 and cyst: Recently, we have shown that loss of Kif3a, a ciliary trafficking protein, also causes impaired lumen formation.28 However, this phenotype was not caused by loss of the cilium but due to misregulated microtubular cytoskeleton in the cell periphery.28 In addition, we found that in early stages of in vitro cyst development where apoptosis-dependent cavitation takes place, cilia are not yet present.28 Therefore, these findings indicate that the ciliary localization of ANO6 may not be a prerequisite for its effect on cyst lumen formation, but may be related to ANO6 located to the plasma membrane.