After cytokine peaks at day 5 (TNF-α and IFN-γ) and day 8 (IL-1β) (Fig 1F to 1H), and a gradual increase of the inflammatory marker, vimentin, in left renal tissue (Fig 1B), a status of CKD is firmly established at day 15 after injury, confirmed by biochemical (Fig 1A), anatomical (Fig 1D) and magnetic resonance scanning imaging (Fig 1E). Here, TNF is linked to chronic kidney disease.