In a healthy population, minute levels of NGAL are filtrated from inside the glomerulus and the luminal NGAL is reabsorbed in the proximal tubule through a megalin-dependent pathway.43 Immediately after AKI, NGAL is upregulated in the ascending limb of the Henle's loop, the distal tubule, and the collecting duct. The gene discussed is LCN2; the disease is acute kidney injury.