The NY-ESO-1157–165 and HLA-A*0201 (HLA-A2) status of different human cancer cell lines: melanoma cell lines: SK-MEL-23 (NY-ESO-1-, HLA-A*0201+), SK-MEL-37 (NY-ESO-1+, HLA-A*0201+), breast cancer cell lines: ALAB (NY-ESO-1+, HLA-A*0201+), MCF7 (NY-ESO-1-, HLA-A*0201+), 734B (NY-ESO-1-, HLA-A*0201+), and multiple myeloma cell lines: ARK (NY-ESO-1+, HLA-A*0201-), U266 (NY-ESO-1+, HLA-A*0201+) was analyzed by flow cytometry and summarized in S1A Fig. This evidence concerns the gene HLA-A and melanoma.