The effect at higher doses on the level of gene expression is further evidenced by the alteration of Tnnc1, Tnnt2, Tnni3, Myh6 and Actn2. Similarly, at increasing (0.1, 0.5, or 1.0 μM) ATO doses the expression of myogenic genes (Myf5, myoD and myogenin) is reduced in mouse embryonal carcinoma P19 cells when induced to differentiate into myoblasts46. This evidence concerns the gene MYOD1 and embryonal carcinoma.