ANXA5 and cancer: The results showed triptolide or celastrol alone treatment caused moderate apoptosis including early (Annexin V positive and PI negative) and late (Annexin V positive and PI postive) apoptosis in both H1299 and H157 cancer cell lines, and co-treatment with triptolide and celastrol caused significant total apoptosis in both cancer cell lines (Figure 4B and 4C).