To investigate the relationship between E6 and hDlg and Cx43 re-location to the cytoplasm in cervical tumour cells, C33a cells were transfected with empty vector (C33aV) or a vector expressing wild type E6 (C33a18E6) or the same vector expressing an HPV18 E6 mutant that does not bind hDlg [27] (Figure 5A). This evidence concerns the gene GJA1 and uterine cervix neoplasm.