Furthermore, multivariate logistic regression analysis showed that high AGK protein expression (P = 0.032), histological differentiation (P = 0.026), and T classification (P = 0.015) were significantly associated with lymph node metastasis in NPC; however, age and gender showed no association with lymph node metastasis (Table 4). This evidence concerns the gene AGK and metastatic malignant neoplasm in the lymph nodes.