NF2 and hepatocellular carcinoma: Reverse transcription PCR (RT–PCR) results revealed that, in addition to wild-type Nf2, there were several additional fragments present in normal liver tissues, HCC cell lines (HCCLM3, Huh-7) and PVTT-derived cells (CSQT2) (Fig. 3b,c), including at least three bands in the HCC tissues and cells (Fig. 3b,c).