However, most PtenΔ/Δ;p110βΔ/Δ animals became moribund at 50–70 days post pIpC with markedly increased thymus weights (Supplementary Fig. 2b), infiltration of terminal deoxynucleotidyl transferase-positive T lymphoblasts (CD4+ or CD4 and CD8 double-positive T-cell blasts) in the thymus and BM and increased white blood cell counts (Supplementary Fig. 2a,c–e), all of which are manifestations of T-ALL. This evidence concerns the gene CD8A and T-cell acute lymphoblastic leukemia.