Blockades of the later phases of autophagy prior to lysosomal fusion may also show apparent increase in autophagic ‘puncta’ or vacuoles while in reality autophagy is impaired.[21] Our experiments using an LC3-GFP-m Cherry construct confirms increased autophagic flux in AML cells with Nutlin 3a treatment. The gene discussed is MAP1LC3A; the disease is acute myeloid leukemia.