In the present study, we found that IL–20 regulated EMT-associated markers (E-cadherin, N-cadherin, vimentin, and fibronectin), affected prostate cell migration, and increased anchorage-independent growth in prostate cancer by inducing the phosphorylation of p38, ERK1/2, AKT, and NF-κB signals. This evidence concerns the gene MAPK3 and Familial prostate cancer.