No effect was observed on CLAUDIN–5, NECTIN protein accumulations (Fig 5C); however we confirmed the impact of cell-cell interactions within the seminiferous tubule as the protein accumulations of INTEGRIN-β1, VIMENTINE, N-CADHERIN and β-CATENINE was decreased in HF-CA exposed males compare to HFD group (Fig 5C). This evidence concerns the gene NECTIN1 and hydrops fetalis.