We observed that the number of cells expressing cleaved and total caspase-3 was significantly reduced in the liver of obese rats treated with OLHHA, which is consistent with previous studies reporting that the development and progression of NAFLD is associated with activation of apoptosis pathways (Feldstein and Gores, 2005; Guicciardi and Gores, 2005). The gene discussed is CASP3; the disease is metabolic dysfunction-associated steatotic liver disease.