To further explore the relationship between RAD17 and the checkpoint kinases, we created stable knockdown cell lines using lentiviral shRNA constructs targeting RAD17 in HeLa cells, a human cancer cell line derived from a cervical adenocarcinoma, as well as LN428, a human cancer cell line derived from glioblastoma multiforme [47]. This evidence concerns the gene RAD17 and glioblastoma.