Specificity of our lead monoclonal antibody, designated PW-3, was confirmed by its detecting only the correctly sized ∼153kD CEMIP protein band in Western blots of FET colon cancer cells that are positive for CEMIP transcript, versus detecting no protein bands in RKO colon cancer cells that are CEMIP transcript negative (Figure 2A). This evidence concerns the gene CEMIP and malignant colon neoplasm.