To determine how PAT4 knockdown might inhibit tumour growth, we analysed mTORC1 signalling in stably transfected HCT116 clones carrying inducible PAT4 shRNAs, shPAT4(4.8) and shPAT4(7.1), and in the non-targeting clone, shNT. Here, SLC36A4 is linked to neoplasm.