CD8A and neoplasm: We studied CD4+ and CD8+ T cell ratios and Treg (identified by CD3+CD4+CD25highFoxP3+) frequencies in both samples by flow cytometry, and found in concordance with our immunohistochemistry data, a higher percentage of CD8+ T cells (46.2% vs. 25.1%) and a lower percentage of CD4+ T cells (48.1% vs. 72.3%) in the tumor area than in the T cell area.