We show here that antigen-spreading during the repeated eliminations of AB1-GAG mesothelioma by sPD1-p24fc/EP vaccinations indeed resulted in the generation of effective tumor-specific cytotoxic CD8+ T cells, which were capable of inhibiting PD1/Tim3 expression on their surface, reducing the number of MDSCs, and rejecting WT-AB1 malignant mesothelioma. The gene discussed is HOXD13; the disease is malignant mesothelioma.