Of particular interest was that there was significant increase in Ad E1A protein in RdB-1L-VSVG tissue whereas E1A was nearly detected in RdB-treated CAR-negative MCF7 tumor, suggesting RdB-1L-VSVG can overcome CAR-dependency for cell entry and replicates actively in CAR-negative tumor tissue. The gene discussed is DHTKD1; the disease is neoplasm.