By biochemical approaches we detected CLIC1 protein in GBM cell culture media; moreover, by directly tracking exogenous CLIC1 protein (i.e. FLAG-tagged form of CLIC1 protein) in vitro, we demonstrated that CLIC1 protein can be secreted by a GBM donor cell and can efficiently enter into a different GBM recipient cell. This evidence concerns the gene CLIC1 and glioblastoma.