Considering that ubiquitination represents a major signal triggering the recruitment of ESCRT machinery and cargo incorporation within EVs [49, 50], the two structural hints existing in CLIC1 protein sequence, together with our biochemical data showing ubiquitinated CLIC1 in GBM cells, foster CLIC1 expression within EVs isolated by human GBM cells. Here, CLIC1 is linked to glioblastoma.