Consistently, immunohistochemical staining analysis of URGCP also demonstrated that URGCP predominantly located in the nucleus of NSCLC tissues at advanced clinical stage (stage III and IV), especially in those with metastatic disease, whereas NSCLC tissues at early clinical stage (stage I and II) mostly expressed URGCP in the cytoplasm (Fig. 2A). This evidence concerns the gene URGCP and metastatic neoplasm.