URGCP and urinary bladder cancer: To determine URGCP/URG4 expression in bladder cancer cells and tissues, qRT-PCR and western blotting assay showed that both mRNA and protein expression of URGCP/URG4 were significantly upregulated in bladder cancer cells, compared to that of in the primary-cultured normal bladder epithelial cells (indicated as NC) (Figure 1A and 1B).