qRT-PCR analysis found the expression of NF-κB-targeted genes, including CCND1, Bcl-2, MMP9 and VEGF were upregulated once overexpression of URGCP/URG4, whereas knockdown of URGCP/URG4 significantly inhibited the levels of these genes in bladder cancer cells (Figure 5E). This evidence concerns the gene MMP9 and urinary bladder cancer.