Therefore, although rapamycin treatment of 4T1 tumor cells can result in high expression of autophagosomal LC3β-II (Fig 3A), the rapamycin-resultant autophagosome preparation had little effect in vivo on activating DC-mediated DC8+ T cell and splenocyte proliferations (Fig 3B and 3C). Here, NSL1 is linked to neoplasm.