To determine if the deafness causing mutation affects the ability to localize to the tips of filopodia, we over-expressed DsRed tagged CIB2WT (Fig 5A) and CIB2R186W constructs of human CIB2 (Fig 5B and 5C) along with non-tagged human Whirlin and GFP-Myosin 15a in COS-7 cells. The gene discussed is MYH14; the disease is deafness.