TAGLN and neoplasm: When the tumors’ pathological features were analyzed, promoter methylation, but not expression, of TAGLN was significantly decreased in LN negative tumors when compared with LN positives (P = 0.0476, Additional file 5: Figure S1A-B) while both expression and methylation levels did not exhibit any significant difference between grades, stages, and hormone statuses in our tumor-normal matched breast tissue panel.