NPC cells were co-cultured with CIK cells at an E:T ratio of 30:1 for 24 h, and the co-culture supernatants were then harvested and assayed for levels of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ by ELISA. The gene discussed is IL4; the disease is nasopharyngeal carcinoma.