KRAS and cancer: To elucidate the role of oncogenic KRAS in autophagy regulation, we expressed FLAG-tagged wild-type KRAS (KRASWT) or mutated KRAS (KRASG13D, KRASG12D and KRASG12V) in two model systems: in the human non-cancer colon NCM460 cell line, after stable infection, and in the yeast S. cerevisiae ras2Δ strain from the pCM184 plasmid, under the control of a tet-off promoter (this strain was chosen to abolish the input of yeast Ras2p, itself involved in autophagy regulation).