Analysis of mRNA levels of CD3e, CD8a, and the Th1-associated markers IFNγRβ, CCR2, IL-2Rα and CCR5 in tumors from differently immunized groups revealed that immunization with the CTB-MUC1-alum-CpG formulation led to significantly increased infiltration of T cells, especially CD8+ T cells, and Th1 cell clone proliferation in tumor tissues. This evidence concerns the gene CD8A and neoplasm.