Because freshly obtained mouse CD4+ T cells incubated with FITC-labeled ALL showed formation of fluorescent patching and capping on cell surface (data not shown), we examine whether cholesterol depletion in lipid rafts affects cell membrane recognition by ALL. For this, CD4+ T cells were treated with MβCD after activation via CD3/CD28 or CD3/ALL for 48 h. The gene discussed is CD28; the disease is acute lymphoblastic leukemia.