To evaluate cell function, CD4+ T cells were simultaneously stimulated by anti-CD3 mAb plus ALL. The results showed similar levels of transcripts for IL-2, TNF-α, TGF-β, and IFN-γ compared to those shown by CD3/CD28-stimulated CD4+ T cells (Fig. 2a). The gene discussed is CD28; the disease is acute lymphoblastic leukemia.