To test this in pancreatic cancer cases, we first transfected BxPC-3 and AsPC-1 cells with a control vector, TAZ, 4SA, or 4SA-S51A, which had an additional point mutation of 4SA in the TEAD-binding domain and lacked TEAD binding [19, 36]. The gene discussed is WWTR1; the disease is familial pancreatic carcinoma.