Grading of cancer cell nuclear staining in blind-coded sections stained with the second monoclonal pERK antibody (E10) confirmed a significant difference between TRG1-3 and TRG4 (Mann-Whitney test: p = 0.015) and the scores for cancer cell nuclear staining correlated positively with both antibodies (Spearman rho = 0.738, p < 0.0001). This evidence concerns the gene NXN and cancer.