Three larger human studies (n = 366 patients, n = 192 patients, n = 2139 patients) similarly found that LGR5 colorectal cancer tumor expression was significantly associated with higher American Joint Committee on Cancer (AJCC) staging, increased Ki67 indices, advanced histological grade, increased depth of invasion, presence of nodal and distant metastases, and was independently associated with compromised overall patient survival [41, 42]. This evidence concerns the gene LGR5 and neoplasm.