Our results provided evidence that MSE might induce apoptosis via caspase-3/7-independent mechanisms in poorly metastatic colon cells (HT-29), and MSE likely induce late stage apoptosis, in part by activating caspase-3/7 (extrinsic pathway), after prolonged (72 h) exposure of metastatic prostate cancer cells to MSE. The gene discussed is CASP3; the disease is prostate cancer.