However, incubation of 50 μg/mL of MSE after a prolonged exposure (72 h) activated caspase-3/7 in metastatic prostate cancer cells (PC-3), P < 0.05 (Fig. S1B), indicating that MSE might induce late stage apoptosis in prostate cancer cells via extrinsic apoptotic pathway. This evidence concerns the gene CASP3 and Familial prostate cancer.