Furthermore, the Annexin-V assay, using FACS, showed up-regulation of Annexin-V in the lung adenocarcinoma cells following transduction with either lenti-mCherry–pri-miR21 snoMEN, or lenti-mCherry–pre-miR21 snoMEN, but not after transduction with the negative control lentiviral snoMEN vector (Fig 5D). The gene discussed is ANXA5; the disease is lung adenocarcinoma.