TP53 and cancer: Induction in the presence of sucrose led to a pronounced loss of viability for both strains, consistent with the efficient expression of sacB. The titratability and tight basal control for the tT/tT system was further demonstrated for a range of clinically relevant proteins, such as the metabolite cancer target MTH1 (37,38), the cytokine Interferon α2a, and the tumour suppressor protein p53 by western blot analysis (Supplementary Figure S9).