To validate the repression of DNMT3B by miR-29b in HCC cells, we created luciferase reporter constructs containing the 300-bp 3′-UTR of DNMT3B (nt 1182–1209 of DNMT3B mRNA) with wild-type or mutated miR-29b-binding site as reported previously (Figure 4A). The gene discussed is DNMT3B; the disease is hepatocellular carcinoma.