Moreover, measurable differences was also observed in the effects of ICD-related protein components in tumor vaccine adjuvants against 4 T1 tumor metastasis and in mouse survival (Fig. 5a, b, c), i.e., HSP and CRT appear to play a primary and secondary role in these activities, respectively, and HMGB1 has limited or low effect. The gene discussed is HMGB1; the disease is neoplasm.