As shown in Fig 3, quantitative Western blot analyses performed on total cell lysates showed that the level of EGFR phosphorylation (Y1068 residue) was substantially reduced by D609 in A431-AD cells at 24h (to about 72%, P = 0.039) and 48h (to about 62%, P = 0.002), while no effect was observed on the total level of EGFR protein. This evidence concerns the gene EGFR and Alzheimer disease.