Inhibition of endogenous NO production with L-NAME in the presence of Perforin-2 also allowed increased intracellular S. typhimurium replication beginning several hours post-infection (Figure 2C) coinciding with the known time period required for onset of NO production (see Figure 2—figure supplement 1) (Mastroeni et al., 2000). The gene discussed is MPEG1; the disease is infection.